In the past 20 years, mounting evidence suggests that inactivation of tumour suppressor genes in human cells is not only responsible for the initiation and progression of cancers but also for the sensitivity of cancers to a variety of therapies. Therefore, understanding the biological and biochemical function of these tumour suppressor genes is highly important for the prevention, diagnosis, prognosis and treatment of human cancers. LATS is a tumour suppressor that has been shown to be involved in various human cancers. However, how loss of LATS causes tumour development is largely unknown. We have identified several novel downstream proteins of LATS that we are proposing to study in this research project. Furthermore, we propose to explore how these proteins mediate LATS-induced tumour suppression. This study has great implication for the future activation of LATS signaling to kill tumour cells.