The most devastating aspect of cancer is the ability of tumour cells to migrate to other sites and establish new colonies or metastases. Cells migrate by extending feet or pseudopodia. We have characterized the molecules, both RNA and protein, that are found in the pseudopodia and have identified four molecules critical to the formation of pseudopodia but also to the ability of tumour cells to migrate. In this grant, we will identify other pseudopod proteins that regulate tumour cell migration and determine whether they are useful prognostic indicators of cancer malignancy and cancer patient survival. We will also try to identify small molecules that inhibit the behavior of the pseudopod proteins which may lead to the development of novel cancer therapeutics.