The Ras proteins enable cells to communicate outside signals to the nucleus. Certain mutations, which permanently activate Ras, can result in the incorrect propagation of intracellular signals, ultimately leading to tumour growth and metastasis. Indeed, approximately 30% of human tumours contain activating mutations in Ras and in specific tumour types this figure can be as high as 90%. Due to the importance of this protein in oncogenesis, numerous cellular and biochemical studies have been carried out in the past, often relying on chemically modified Ras substrates. Our preliminary data suggest that this work has suffered from the use of non-natural compounds. In the present study, we propose to utilize state-of-the-art NMR spectroscopy and natural substrates to perform real time assays monitoring Ras protein activity. With these methodologies, we hope to gain a better understanding of Ras signaling and the effect of Ras-binding proteins on its structure and activation state.