My research program is focussed on the study of molecular mechanisms implicated in the estrogen receptor-dependent gene transcription in breast cancer cells.  Estrogen plays important roles in both the normal and pathological states in many tissues, including the mammary glands and reproductive tract. The biological effects of estrogens are mediated through two forms of nuclear estrogen receptors (ER) named ERα and β. These receptors control gene expression in conjunction with coregulatory proteins as ligand-regulated transcription factors.  In this research project, we are particularly interested in elucidating the role of the histone H3 lysine 27(H3K27) demethylase JMJD3 in gene expression.  We will use molecular and cellular biology techniques in conjunction with biochemical tools that will help to unveil the functions of JMJD3, as well as the methylation state of H3K27, in the regulation of ER-target genes and breast cancer development.  The results obtained from these studies should lead to new insight that will be directly relevant to the understanding, treatment and prevention of cancer.